How to deal with the pollution of pipette gun in PCR room

1. Clean the waste liquid box and soak the waste liquid box with disinfectant with available chlorine content of 2000mg/L to eliminate nucleic acid pollution; 2. Scrub the pipette with disinfectant water with available chlorine content of 2000mg/L, and the key parts are the parts of the front end of the pipette that are easily contaminated, and then wipe it with clear water after cleaning; 3. Soak the centrifugal pipe rack, the amplification pipe rack, the bottom plate of the amplification pipe rack, the suction nozzle box and other related items in sterile water with the available chlorine content of 500mg/L for 2 hours, then rinse them with clean water and dry them before use; 4. Equipment for repeatedly treating polluted areas with nucleic acid aerosol pollution scavenger, such as amplification instrument and automatic extractor; For biological safety cabinets and other equipment containing air filtration system, it is necessary to replace the filter screen; 5. Clean the whole laboratory (floor, walls, ceiling, doors, windows, etc.). ) Spray disinfection with disinfectant containing 500mg/L available chlorine for laboratory use (the key part is the operation area), effectively degrading aerosol in the air and nucleic acid pollution attached to the surface of the experimental platform; At the same time, increase ventilation (internal circulation is useless, and external circulation as much as possible); 6. After the contaminated area and equipment are cleaned up, the shared equipment and operating table shall be irradiated with a mobile ultraviolet disinfection vehicle for 1 hour; In the laboratory, the indoor ultraviolet lamp is used to illuminate all night. Step 1- 6 must be carried out every day to ensure the elimination of laboratory pollution; The negative control group is designed according to the judgment standard of pollution elimination, and the pollution situation is confirmed according to the negative control. Negative control materials placed in the operation area overnight after opening can be used for experiments (verified in a separate laboratory). If the negative control does not take off for three consecutive days, it means that the pollution is controlled and the normal experiment can be resumed.