PCR laboratory can be divided into sample preparation, nucleic acid diffusion, product analysis of the 3 major areas, each area of the equipment used is different, the following will give you a detailed introduction to each area of the PCR laboratory need what equipment?
Sample Preparation Area:
Biological Safety Cabinet: Prevents the spread of harmful suspended particles and aerosols
Sample Cryogenic Refrigerator: -20 degrees Celsius, -80 degrees Celsius to preserve samples and DNA
High-speed Benchtop Freeze Centrifuge: Collects microbial organisms, cellular debris, and other sediments. Some samples are less stable at room temperature and need to be kept at low temperatures
Water or metal baths: for temperature control of water baths during DNA hybridization
Pipettes: to accurately measure a specific volume of solution
Ultraviolet lamps or sterilizing carts: to sterilize the space environment
Mixer: to mix the samples prior to pipetting
Ultra-clean benches: for all operations involving bacteria, the ultra-clean bench should be used for the collection of microorganisms and DNA. Bacterial operations are done under the ultra-clean bench
Cryogenic shaker: used for shaking culture of E. coli and yeast
Magnetic stirrer: accelerates the dissolution of solid contents
Nucleic acid diffusion zone:
Gene amplifier: DNA/RNA amplification of gene detection
Fluorescent quantitative PCR instrument: nucleic acid diffusion zone: DNA/RNA amplification of gene detection
Fluorescent Quantitative PCR: quantitative analysis of nucleic acids
Nucleic acid extractor: sample DNA/RNA extraction
Pipette: accurate measurement of a specific volume of solution
Ultraviolet lamp or disinfection trolleys: environmental disinfection of the space
Ultra-clean workbench: the operations involving the bacterial need to be completed in the ultra-clean workbench
Water purification unit: for PCR, DNA sequencing requires ultrapure water
Pure water unit: for PCR, DNA sequencing requires ultrapure water. Ultra-pure water is required for DNA sequencing
Product analysis area:
Electrophoresis: Horizontal electrophoresis is used for nucleic acid DNA/RNA detection, vertical electrophoresis is used for protein detection, and transfer electrophoresis is used for transferring proteins to membranes for weatern detection
Gel imaging system/Ultraviolet detector: used for nucleic acid agar Electrophoresis and protein polypropylene amide observation and photography; UV analyzer for stained nucleic acid agar electrophoresis gel observation, can not be connected to the computer
Electric furnace: electrophoresis agar gel heating and melting