Is it normal for nucleic acid test to be negative (analyze the accuracy and limitation of nucleic acid test results)

Nucleic acid detection is a method of detecting virus nucleic acid, which can detect whether it is infected with virus. The principle of nucleic acid detection is to collect virus nucleic acid from patient samples, then amplify it by PCR amplification technology, and finally detect the existence of nucleic acid by fluorescent probe.

Accuracy of nucleic acid detection

The accuracy of nucleic acid detection depends on many factors, including the time, place, method and detection method of sample collection. Under the correct operation, the accuracy of nucleic acid detection can reach more than 95%.

Limitations of nucleic acid detection

Although the accuracy of nucleic acid detection is high, it still has some limitations. First of all, nucleic acid detection can only detect whether the virus nucleic acid exists, but not whether the virus is active. Secondly, the results of nucleic acid detection may be affected by the time, place and method of sample collection. Finally, the variation of virus may lead to false negative or false positive results of nucleic acid detection.

Is nucleic acid test negative normal?

Under the correct operation, a negative nucleic acid test usually means that the patient is not infected with the virus. However, the results of nucleic acid detection may be influenced by many factors, such as the time, place and method of sample collection. In addition, the variation of virus may lead to false negative results of nucleic acid detection. Therefore, when conducting nucleic acid detection, we should pay attention to the time, place and method of sample collection, and make a comprehensive judgment based on clinical manifestations and other test results.

Operating steps of nucleic acid detection

1. Sample collection: The samples for nucleic acid detection can be nasopharyngeal swab, throat swab, saliva, sputum, feces, etc. Wash your hands and wear masks and gloves before collection. Attention should be paid to avoid pollution and contact with other personnel when collecting.

2. Sample treatment: put the collected samples into test tubes, add protective solution, shake well and send them to the laboratory.

3. Nucleic acid extraction: the nucleic acid in the sample is extracted to remove impurities.

4.PCR amplification: put the nucleic acid into a PCR amplifier for PCR amplification.

5. Fluorescent probe detection: PCR amplification products are combined with fluorescent probes to detect whether there is virus nucleic acid.