In order to meet the demand of rapid detection in the field, hand-held ATP fluorescence detector (also called hand-held ATP fluorescence detector) should be selected to realize real-time detection of flow state, because large desktop ATP fluorescence detector may be better than hand-held ATP detector in sensitivity, but it can only be used in laboratory, which is not conducive to on-site detection in mobile places such as catering service and medical control.
ATP detection technology abroad has been studied since 1990s, and has made great progress and development so far.
The experimental results of ATP detection technology are accurate, reliable and credible. Over 65,438+0,000 samples have been studied by many professional authorities in the United States. This rapid detection method can complete the detection in just over ten seconds, and the consistency between the experimental results and the results obtained by 24-48 hours standard bacterial culture method/plate method is over 90%. When detecting drinking water, the sensitivity is more than 100 times higher than that of the standard method, and the sample size is only1100 of that of the traditional method. Compared with the results obtained by traditional bacterial culture, the correlation of this product is as high as 92-98%. The Canadian Food Inspection Association found that the correlation between the determination results of this product and the standard culture method was 98%. FDA recommended ATP detection system and kit for rapid detection of bacteria in milk. The first Sino-US seminar on food safety and its whole process control and the training course on rapid automatic microbial detection technology also took ATP rapid detection technology as one of the training contents. At the 1 1 World Disinfection Conference and the 7th International Disinfection and Hospital Infection Control Forum held in Brazil, the participating experts made a research report on the application of ATP method in the evaluation of cleaning efficiency. The conclusion is that the effect of this method is basically the same as that of the standard culture method, so ATP method is included in the relevant standards of "medical cleanliness evaluation" in many countries as one of the evaluation methods. Through application and evaluation, the "Guidelines for Disinfection and Sterilization of Medical Institutions 2008" formulated by CDC in the United States also uses ATP method as a new method to evaluate the effect of endoscopic cleaning or endoscopic treatment. In recent years, due to the domestic application demand, leading units have developed hand-held ATP fluorescence detectors and ATP reagents with independent intellectual property rights, which have been successfully applied in many fields. China CDC has confirmed that ATP test results are linearly related to different concentrations of Escherichia coli, Staphylococcus aureus and blood. Through the application and technical evaluation of ATP method for bacteria detection and surface cleanliness detection, ATP fluorescence detector has become a special detection equipment related to health supervision and food safety designated by China's health supervision and China's food and drug supervision official department, and has been designated by the state for rapid detection of surface cleanliness of objects and operators in food processing, storage and transportation, trade, catering service and medical system, and it is an important measure for building the capacity of health supervision and rapid detection in the field of food safety to purchase ATP fluorescence detector and supporting reagents. In China, the development of hand-held ATP fluorescence detector began around 2005. On 20 10, the first hand-held ATP fluorescence detector and an integrated swab for on-site express detection of ATP reagents were developed, and the corresponding invention patent for the first hand-held ATP fluorescence detection system in China was obtained. With the expansion of demand and the gradual maturity of products, the strong sales growth of domestic instruments has gradually replaced the share of imported instruments.