The most important performance indicator is its safety.
When the laboratory level for the first class generally do not need to use a biological safety cabinet, or the use of class I biological safety cabinet. Laboratory level for the second level, when the operation may produce microbial aerosols or spills, you can use Ⅰ biological safety cabinet; when dealing with infectious materials, should use part or all of the exhaust Ⅱ biological safety cabinets; if it involves the handling of chemical carcinogens, radioactive substances and volatile solvents, you can only use the Ⅱ - B level of full-exhaust (B2-type) biological safety cabinets.
When the laboratory level is three, should use class II or III biological safety cabinet; all operations involving infected materials, should use the full exhaust type Ⅱ - B class (B2 type) or Ⅲ biological safety cabinet. When the laboratory level is level IV, class III fully-ventilated biological safety cabinets should be used. When the personnel wearing positive pressure protective clothing, can use Ⅱ - B class biological safety cabinet.
Extended information
In order to ensure the safety of biological safety cabinets, safety cabinets should be regularly maintained and serviced:
1, each time before and after the use of safety cabinets should be cleaned and disinfected work area.
2, HEPA filter service life expires, should be replaced by professionals who have received special training in biological safety cabinet.
3, WHO issued a laboratory biosafety manual, the U.S. biosafety cabinet standard NSF49 and the China Food and Drug Administration biosafety cabinet standard YY0569 require one of the following conditions, should be biosafety cabinets for safety testing: before the installation is completed and put into use; a yearly routine testing; when the cabinet is shifted; the replacement of HEPA filters and internal components after maintenance. components after maintenance.
Safety testing includes the following aspects:
1, inlet air flow and wind speed detection: inlet air flow using the smoke method or wire method in the work of section detection, detection of the location of the working window, including the edges of the perimeter and the middle of the window area; inlet air flow wind speed using an anemometer to measure the work of the window section of the wind speed.
2. Sinking airflow wind speed and uniformity detection: the anemometer is used to measure the cross-section wind speed with uniform distribution points.
3, the work area cleanliness detection: using dust particle timer in the work area detection.
4, noise detection: the front panel of the biological safety cabinet horizontal center outward 300mm, and higher than the work surface 380mm at the sound level measurement noise.
5, illumination detection: every 30cm along the length of the work surface of the center line to set a measurement point.
6, box leakage detection: to the safety cabinet sealed and pressurized to 500Pa, 30 minutes after the test area connected to a manometer or pressure sensor system to detect the pressure attenuation method, or with soap bubble method.