There are environmental requirements, personnel requirements, and sterile goods expiration date requirements.
Environmental requirements?
1, sterilized items should be stored in the sterile storage room storage rack or storage cabinet, storage rack or storage cabinet should be easy to clean, not easy to rust; save the environment should be clean, bright, ventilated or air purification devices, lighting light; temperature below 24 ℃, humidity below 70%.
2, sterilized items should be classified and stored, disposable sterile items should be removed from the outer layer of the large packaging storage, storage location is relatively fixed, clearly marked; items should be stored from the ground 20 ~ 25cm, from the wall 5 ~ 10cm, from the ceiling 50cm.
3, operating rooms, treatment rooms, dressing rooms, etc., the use of natural ventilation, when the ventilation is poor, you can use the exhaust fan to force the ventilation; sterile goods storage environment should be clean, bright, ventilated or have adequate air purification devices; temperature below 24 ℃ humidity below 70%. Ventilation; sterile goods preservation environment should be cleaned daily, the object surface and the ground wet wiping to avoid dust.
4, the establishment of work records.
Personnel requirements
1, sterile goods storage area should be responsible for the management of a person; hand washing before contact with sterile goods.
2, the inventory of items to the main visual inspection, reduce the touch.
3, the sterile goods should be placed in order of expiration date, the expiration date sign is conspicuous, near the expiration date of the goods on the location of easy access; single-use sterile goods should be a batch of finished and then put into the next batch, or a small amount of the remaining unused batch of goods on the upper level.
Aseptic articles expiration date
1, aseptic articles preservation of the validity of no seasonal restrictions, according to the different packaging materials preservation of different expiration dates, the use of the expiration date should be carefully examined signs.
2, the packaging of sterilized items should be neat and not easy to loose, good sealing, no holes, sterilization date and expiration date mark clearly, in accordance with the order of expiration date sequential code in the storage rack or storage cabinets, expiration date markers should be clear, easy to visually count.
Extended information:
Sterilization of the commonly used methods of sterilization chemical reagents sterilization, ray sterilization, dry heat sterilization, wet heat sterilization and filter sterilization. Can be based on different needs, using different methods, such as media sterilization generally use moist heat sterilization, air is used to filter sterilization.
1, heat sterilization method
Heat sterilization method using high temperature to make all the proteins within the microbial cell denaturation, enzyme activity disappears, resulting in cell death. Usually dry heat, moist heat and intermittent heating sterilization method.
2, dry heat sterilization
Flame burning method or oven hot air sterilization method called dry heat sterilization (dryheatsterilization).
The metal instruments or washed glassware into the electric oven, maintained at 150 ~ 170 ℃ for 1 ~ 2 hours, can achieve complete sterilization (including bacterial spores). Cauterization (incineration or combustion) is one of the most thorough dry heat sterilization method, the scope of application is limited to the inoculation ring, inoculation needle sterilization or with pathogenic materials, animal carcasses, such as burning.
3, moist heat sterilization
Boiling water, steam and steam pressure sterilization.
Pasteurization: because the earliest by the French microbiologist Pasteur for fruit wine sterilization, so the name. It is a low-temperature sterilization method dedicated to liquid flavored foods or seasonings that should not be sterilized at high temperatures, such as milk, beer, fruit wines or soy sauce.
Boiling disinfection method: the use of boiling at 100 ℃ for several minutes, generally used for the disinfection of drinking water.
4, intermittent sterilization
Intermittent sterilization for three consecutive days, a daily steam sterilization method. This method is applicable to the material and some sugar or protein substances that can not withstand temperatures above 100 ℃. Generally in the normal atmospheric pressure with steam sterilization for 1 hour. Sterilization temperature does not exceed 100 ℃, does not cause the destruction of sugar and other substances, but can be intermittent culture during the germination of spores to kill, so as to achieve the purpose of complete sterilization.
5, radiation sterilization
Radiation sterilization in certain conditions using rays for sterilization methods. More commonly used are ultraviolet light, and other ionizing radiation (ray accelerated neutrons, etc.). Wavelength of 25000 ~ 80000 nanometers between the laser also has a strong ability to sterilize the wavelength of 26500 nanometers is most effective. Radiation sterilization method is limited to a certain material, due to the complexity of the equipment required, difficult to use widely.
6, osmotic pressure sterilization
Osmotic pressure sterilization using high osmotic pressure solution sterilization method. In a high concentration of salt or sugar solution in the cell due to dehydration and plasma wall separation, can not carry out normal metabolism, resulting in the death of microorganisms.
7, chemical reagents sterilization
Most of the chemicals in the low concentration of bacteriostatic effect, high concentration of bactericidal effect. Commonly used 5% carbolic acid, 70% ethanol and ethylene glycol. Chemical sterilization agent must have volatile, in order to remove the residual drug on the material after sterilization.
Chemical sterilization of commonly used reagents are surface disinfectants, antimetabolites (sulfonamides, etc.), antibiotics, biopharmaceuticals antibiotics are a class of microorganisms or other biological life activities in the process of the synthesis of secondary metabolites or artificial derivatives.
They can be used as excellent chemotherapeutic agents because they can inhibit or infect the life activities of other organisms (including pathogenic bacteria, viruses, cancer cells, etc.) at very low concentrations.
Reference: Baidu Encyclopedia - Sterilization