A, take material
The main thing is to receive the specimen, in the taking table for each specimen to carefully check, including the patient's name, gender, age, registration number, the name of the specimen, the site, the patient's medical history, the operation of the seen, and so on. For large specimens such as stomach, intestines, and uterus will be incised to initially determine the site of the lesion and fixed with a 10% neutral formalin (formaldehyde) solution for 6-48 hours, also called incisional fixation.
After fixation, the doctor will select the lesion site based on the patient's auxiliary examinations, especially imaging results, combined with the patient's clinical history, and cut an area no larger than 2*1.5*0.3 cm. According to the patient's degree of progression of the disease, the number of pieces of tissue taken from each specimen also ranges from 1 to 100.
Second, dehydration
When all the specimens are taken, the technician and the doctor who took the specimen check carefully, and then the technician puts the taken tissues into the dehydrating machine to carry on the dehydration, transparency, and wax dipping procedure for 15 hours.
Three, embedding
After dehydration, the embedding of the tissue begins. Embedding is carried out on an embedding machine, which has a 60°C table and a -10°C table, and is topped with melted paraffin wax, which is pressed out of the wax flow nozzle. Take an embedding cassette, add some paraffin wax, then secure the tissue with forceps and move it to the -10°C table, the paraffin solidifies quickly and the tissue is secured inside, and finally put on the well-marked lid. It is placed on the freezing table and the embedding box can be removed after about half an hour.
Fourth, sectioning
In clinical pathology and histology laboratories, sectioning is one of the most important techniques, the use of pathology sectioning machine to cut each block of wax into thin slices of 1-6 microns in accordance with the regulations.
V. Spreading, baking, staining, sealing
To be complete and wrinkle-free, paste on the appropriate slide baking, this process most hospitals will use the spreading and baking machine (or spreading machine, baking machine), and then stained with staining machine staining, sealing machine sealing, but also directly using the sealing and staining machine for staining and sealing.
Sixth, diagnostic reading
From the beginning of the embedding to the end of the sealing film about 6-8 hours, to here, the tissue specimen preparation is complete, you can wait for the diagnostic room reading and report. After the reading is completed, the pathology online report can be issued through the pathology graphic reporting system for patients to remotely query the pathology results.