2 Monitoring standard of disinfection and sterilization effect in operating room 1. What is the qualified rate standard of disinfection and sterilization in operating room? All articles disinfected by pressure steam, boiling, chemical liquid immersion and fumigation, such as instruments, dressings, rubber, glass, stainless steel or enamel cans and boxes, should reach 100%.
2. Operating room air disinfection standard
(1) The number of air bacteria (cfu/m3) in the first-level laminar flow clean operating room should be ≤ 10, the surface of the object should be ≤5, and the operator's hand should be ≤5.
(2) The number of air bacteria (cfu/m3) in the secondary general operating room should be ≤200, the surface of the object ≤5, and the operator's hand ≤5.
(3) No pathogenic bacteria, such as hemolytic streptococcus B, Staphylococcus aureus, Salmonella, etc. , can be detected in the air of class I and II operating rooms.
3 Common methods for monitoring the sterilization effect of pressure steam? Refer to the disinfection supply room procedure.
4 The sampling standard of sterile articles in the operating room should be under strict aseptic technical operation. Take 2 samples, cut off about 1cm×3cm sterile dressing and various catheter samples with sterilization scissors, and inoculate them into 40ml aerobic, anaerobic and fungal culture media respectively. For special medical consumables that cannot be sampled by destructive methods, foam can be coated on the surface of cotton swabs for sampling.
5 standard cotton swab sampling method for object surface sampling in operating room: within 4 hours after disinfection and sterilization. Soak 1 cotton swab in sterile saline sampling solution, smear it in a standard sterilization standard plate of 5cm×5cm for 5 times, continuously sample the area of 14 standard plate, cut off the hand contact part, and put the cotton swab into a test tube filled with 10ml sampling solution for inspection. Calculate the result according to the formula.
6 air sampling specification 1. Plate sedimentation method? After disinfection, before operation, put the ordinary nutrient agar plate with a diameter of 9cm on the sampling point in the operating room. There should be three sampling points in the operation room below 30㎡ (a diagonal line is set, and three points are taken from the top, that is, the center point, and both ends are a little away from the wall at 1m), and five points are set above 30㎡ (east, west, south, north and middle, including east and middle). 0.8m 1.5m above the ground, open the plate cover, place it next to the plate, expose it for 5min, cover it after sampling, and send it to an incubator at 37℃ for 24h to observe and calculate the average colony number of the plate.