The main equipment, reagent preparation area and instruments of PCR laboratory mainly include refrigerator, ultra-clean table, sampler, mixer, balance and centrifuge. In addition to special use, samplers and balances should also be calibrated regularly. Reagent subpackaging should be carried out in an ultra-clean workbench, and the amplification reaction mixture should be of molecular biology grade. In the specimen preparation area, instruments and equipment mainly include biosafety cabinets, samplers, centrifuges, incubators and mixers. The following is the sample preparation area of PCR laboratory. In the amplification area, the main instruments are nucleic acid amplification instrument, ultra-clean table, micro sampler, centrifuge and movable ultraviolet lamp. The amplifier needs calibration. It is also equipped with UPS power supply to prevent power failure caused by voltage fluctuation from affecting the amplification effect. The main instruments and equipment in the analysis area are enzyme-labeled instrument, cleaning machine, sampler and water bath box, and the area of the buffer room in the clean experimental area shall not exceed one-eighth of the main laboratory, which is stipulated.
The pcr laboratory belongs to the third-level biosafety laboratory. Pcr laboratory is also called gene amplification laboratory. PCR is the abbreviation of polymerase chain reaction. It is a molecular biology technique used to amplify specific DNA fragments, which can be regarded as special DNA replication in vitro. Through the DNA gene tracking system, we can quickly grasp the virus content in patients, and its accuracy is as high as nanometer level.
Summary of knowledge points of licensed pharmacist examination chapters, I wish you easy evidence collection.
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