High-speed countercurrent chromatography (HSCCC) is a continuous high-efficiency liquid-liquid partition chromatographic separation technique developed in the 1980s, which does not use any solid support or carrier. It utilizes a two-phase solvent system to establish a special unidirectional hydrodynamic equilibrium in a high-speed rotating spiral tube, in which one phase serves as the stationary phase and the other phase serves as the mobile phase, and a large amount of the stationary phase can be retained during the continuous elution process.
Because there is no need for a solid support body, the separation of substances is realized according to the difference in their partition coefficients in the two phases, thus avoiding the loss of samples, inactivation, denaturation, etc., caused by irreversible adsorption, and not only making the samples able to be fully recovered, but also the recovered samples can better reflect their original properties, especially suitable for the separation of natural bioactive ingredients. Moreover, because of the full contact between the separated substances and the liquid stationary phase, the amount of sample preparation can be greatly improved, which is an ideal means of preparative separation.
In comparison with the traditional solid-liquid column chromatography, it has the advantages of wide range of application, flexible operation, high efficiency, rapidity, large amount of preparation, and low cost, etc. HSCCC technology is developing into a new type of separation and purification technology that attracts much attention, and it has been widely used in the fields of biomedicine, natural products, food, and cosmetic products, and has been recognized as an effective and efficient separation and purification technology in the natural product industry, especially in the natural product industry. It has been widely used in the fields of biomedicine, natural products, food and cosmetics, especially in the natural products industry, and has been regarded as an effective new separation technology; it is suitable for the separation and purification of small and medium molecules.
China is the first country to carry out countercurrent chromatography after the United States and Japan, and Russia, France, Britain, Switzerland and other countries have also carried out this research. The U.S. FDA and the World Health Organization (WHO) have cited this technology as the separation and determination of antibiotic components, and since the 1990s, high-speed counter-current chromatography has been widely used in the separation and preparation of natural drug components and analysis and determination.
4. Examples of applications Here is a brief list of some preparative chromatographic literature using countercurrent chromatography
Research of chemical constituents in He Shou Wu using high-speed countercurrent chromatography
Use of high-speed countercurrent chromatography in the preparative separation and purification of five compounds in Chinese traditional Chinese medicine Tiger Balm
Use of high-speed countercurrent chromatography in the preparative separation and purification of Coumestrol in Codonopsis pilosula
Use of high-speed countercurrent chromatography in the preparative separation and purification of Coumestrol in Codonopsis pilosula. Preparative isolation and purification of coumarin components in Angelica sinensis by high-speed counter-current chromatography
Preparative isolation and purification of coumarin active components in Piper betel
Preparative isolation and purification of psoralen and isopsoralen in psoralen by high-speed counter-current chromatography
Preparative isolation and purification of psoralen and isopsoralen in Qinpi by high-speed counter-current chromatography
Preparative isolation and purification of psoralen in Qinpi by high-speed counter-current chromatography
Preparative isolation and purification of two coumarins in Thymus vulgaris using high-speed counter-current chromatography
Preparative isolation and purification of gimadinone and curcumadione in turmeric rhizome oil using high-speed counter-current chromatography
Preparative isolation and purification of lignan lactone and dehydro Orthogonal experimental design for supercritical fluid extraction of Cirsium cinnamomi
Supercritical fluid extraction of 7-methoxy-daphnoretin and 1,5-diphenyl-1-pentanone from Cirsium cinnamomi wolfsbane by high-speed counter-current chromatography
Preparative separation and purification of coumarin dimethyl ether from C. sinensis by high-speed counter-current chromatography