A marked label; ?
2 mL spiral tube (outer spiral) contains mobiobuffer 750 μ l; ?
Sterile sampling swab; ?
Wet the swab with sterile SCF- 1 solution (50mMTris buffer [pH 7.6], 1 mMEDTA [pH 8.0], 0.5% Tween-20) before sampling; ?
Zipper plastic bags are used to collect sampling tubes; ? Portable ice box (containing ice cubes).
Second, the skin survey
1. The object exclusion criteria are as follows:?
① During the first seven days, the subjects used antibiotics or steroids locally on the face, neck, arms or hands. (Screening and sampling can be postponed when the subject meets the exclusion period of antibiotic or steroid use); ?
② Acne should not appear on face, chest, back and shoulders; ?
③ There are many blisters, pustules, burns, abscesses, erosions or ulcers on the scalp, face, arms, forearms or hands; ?
④ A single blister, pustule, scald, abscess, infection, ulcer, scab, incision, crack or erythema/pigmentation spot or plaque is within 4 cm from the sampling point; ?
⑤ Multiple pink erythema/red scaly spots/plaques (psoriasis or eczema) in any part of the body; ?
⑥ Even thickened, cracked and excessively dry dead skin appears on both sides of palm and/or sole; ?
⑦ Use an over-the-counter dandruff shampoo with unknown ingredients for 2 weeks every day; ?
(8) Diffuse rash (appearing in many parts of the body or spreading to most areas of the body).
2. Test conditions:?
Skin examination should be carried out under good lighting conditions, including general survey of local skin (whole body) and detailed examination of sampling area. ?
3. Test additional standards:
① Carefully visually check whether there are wrinkles in the back ear (behind the left and right ears) and the elbow socket (inside the elbow) of both arms and elbows; ?
② Individuals with any single blister, pustule, scald, abscess, infection, ulcer, scab, incision, crack or erythema/pigmentation spot or plaque can be seen, and those who are more than 4 cm away from the sampling site are still considered eligible for inclusion in the study.
Third, the sample collection method
Description:
① All samples should be collected by aseptic technique (see appendix for details).
② Wear a pair of sterile latex gloves only when collecting skin samples from one subject, and pay attention to avoid glove contamination. ?
(3) If the gloved hand is used to stretch the skin for sampling and contact with the sampling area, the gloves must be replaced before sampling at the next position.
Sample collection order:
① Wrinkles behind the ear (collected separately from the left and right sides behind the ear)?
② Elbow fossa (collected from the inner elbow and the left and right sides respectively)?
③ Anterior nostril (left and right sides can be collected together)
Note: the sampling process is more conducive to the subjects lying down comfortably.
1.？ Posterior auricular fold
Posterior ear fold: refers to the place where the top of the ear connects with the face and extends to the place where the earlobe connects with the face.
① Collect skin samples with a sample collection swab and moisten them with SCF- 1 solution. ?
② To reach this area, please fold your ear forward with one hand to reveal the crease. ?
③ With the other hand, keep the axis of the cotton swab parallel to the skin surface, along the direction of anti-wrinkling, and exert a steady force (wiping for 50 times in about 30 seconds). ?
(4) Insert the cotton swab head into the collection tube, cut the cotton swab head aseptically from the handle, and rotate the tube cap back to the correct position.
note:
In order to obtain the best skin surface specimen, the key points of sampling technology are: using wet cotton swab, applying stable pressure and consistent friction (sampling point goes back and forth 50 times in 30 seconds). ?
Samples were taken from the left side and the right side respectively, and marked and stored separately. Put the test tube in a self-sealed bag with zipper and put it on ice. Send it to the laboratory for preservation within 2 hours.
2. Elbow socket (inner elbow)
Elbow fossa (inner elbow): refers to the joint between the arm and forearm, just at the bend of the inner elbow.
① Collect skin surface samples with a sample collection swab and moisten them with sterile SCF- 1 solution.
② Stretch the elbow skin with one hand, hold the cotton swab with the other hand, make the axis parallel to the skin surface, and wipe the cotton back and forth along the wrinkles in front of the elbow for 50 times, with steady force (50 wipes are completed in about 30 seconds).
③ Insert the swab into the test tube, insert the swab head into the collection test tube, cut the swab head aseptically from the handle, and screw the test tube cover back to the correct position.
note:
In order to obtain the best skin surface sample, the key points of sampling technology are: using wet cotton swab, applying stable pressure and consistent friction (sampling point goes back and forth 50 times in 30 seconds).
Samples were taken from the left side and the right side respectively, and marked and stored separately. Put the test tube in a self-sealed bag with zipper and put it on ice. Send it to the laboratory for preservation within 2 hours.
3. Anterior muzzle
① Wipe the nasal mucosa gently with sterile cotton swab, and wipe each nostril twice with the same cotton swab at the same place. ?
② Take left and right nostril samples as joint samples for sampling and collection, and mark them.
③ Immediately after wiping, put the cotton swab into 750μ biological buffer and rotate it. Keep the swab sponge on the tube wall for more than 20s to ensure that the bacteria are transferred into the solution.
Put the sample collection tube into a zipper self-sealing bag and put it on ice, and transfer it to the laboratory for processing within about 2 hours.
Four. Additional record
aseptic technique
Principle:
All samples were collected and processed by aseptic technique.
All collected materials in each SOP are guaranteed to be clean and sterile or confirmed sterile before purchasing and using.
The following are suggestions for aseptic technology. ?
Aseptic technology operation is suitable for all sample collection SOPs.
Aseptic technology-Preparation of sample collection tubes
1) Sterile articles include (but are not limited to) test tubes, gun heads, MoBiobuffer and SCF- 1 buffers.
2) If the pipeline is to be filled, MoBioBuffer and SCF- 1 will be packed in a laminar flow hood.
Aseptic technology-subject sample collection
1. Handle biological samples according to all safety guidelines of local safety committees and/or institutional policies. Gloves, safety glasses or masks and laboratory clothes must be worn during the whole process of repackaging serum. Precautions should be taken when collecting any biological samples, including at least wearing gloves. ?
2. When handling the sample collection tube during sampling, attention should be paid to keeping gloves clean to avoid cross-contamination inside and outside the collection tube. ?
3. In the process of sample collection, use clean disposable gloves. ?
A) When collecting skin and nasal cavity samples, collect four skin parts before collecting nasal cavity samples. As long as the skin is stretched without touching the sampling area, the sampler can complete the sample collection of all skin parts with a pair of gloves. Once in contact with the sampling area, the next skin sample should be collected after changing gloves.
B) Researchers can use a pair of gloves to collect samples from different parts of the same subject's mouth. Similarly, when the same subject collects three vaginal samples, there is no need to change gloves.
Aseptic technology-sample testing
Sterile supplies include (but are not limited to) test tubes, gun heads and all buffers/reagents.