1, the stationary phase of the column, that is, the model of the column;
2, the specifications of the column, that is, the column length of the column, the inner diameter of the column, the thickness of the film of the stationary solution, and other factors;
3, the type of carrier gas;
4, the flow rate of the carrier gas, the column effect of the different flow rates are not the same;
5, column temperature;
6, the structure of the injection chamber;
7, the structure of the detector.
Separation, also known as resolution, in order to determine the separation of separated substances on the column in the column, commonly used as an indicator of the total separation efficiency of the column. R is equal to the ratio of the difference between the retention time of the adjacent peaks and the average value of the peak widths of the two peaks. The ratio of the difference between the retention time of two neighboring peaks to the average peak width. It is also called resolution, which indicates the degree of separation of two neighboring peaks.The larger R is, the better the separation of two neighboring components. Generally speaking, when R＜1, the two peaks are partially overlapped; when R=1.0, the separation degree can reach 98%; when R=1.5, the separation degree can reach 99.7%. R=1.5 is usually used as a sign that the two neighboring components have been completely separated. When R=1, it is called 4σ separation, the two peaks are basically separated, the bare peak area is 95.4%, the inner peak base overlap is about 2%.R=1.5, it is called 6σ separation, the bare peak area is 99.7%.R≥1.5 is called complete separation. The Chinese Pharmacopoeia stipulates that R should be greater than 1.5. The formula for calculating the degree of separation: R=2(tR2-tR1)/(W1+W2)